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Oxytocin Receptor Rabbit mAb (A19740)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Oxytocin Receptor Rabbit mAb (A19740)

Western blot analysis of various lysates using Oxytocin Receptor Rabbit mAb (A19740) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameOxytocin Receptor Rabbit mAb
Catalog No.A19740
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2266
The protein encoded by this gene belongs to the G-protein coupled receptor family and acts as a receptor for oxytocin. Its activity is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. The oxytocin-oxytocin receptor system plays an important role in the uterus during parturition.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human Oxytocin Receptor (P30559).
SequenceLLLALSGNACVLLALRTTRQKHSRLFFFMKHLSIADLVVAVFQVLPQLLWDITFRFYGPDLLCRLVKYLQVVGMFASTYLLLLMSLDRCLAICQPLRSLRR
Gene ID
Swiss Prot
SynonymsOTR; OT-R; Oxytocin Receptor
Calculated MW43kDa
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, PC-3, Jukat, Mouse brain
Cellular locationCell membrane.
Customer validation

IHC(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Oxytocin Receptor Rabbit mAb (A19740)}

Western blot - Oxytocin Receptor Rabbit mAb (A19740)

Western blot analysis of various lysates using Oxytocin Receptor Rabbit mAb (A19740) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)