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PDE4A Rabbit pAb (A20162)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PDE4A Rabbit pAb (A20162)

Western blot analysis of various lysates using PDE4A Rabbit pAb (A20162) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - PDE4A Rabbit pAb (A20162)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using PDE4A Rabbit pAb (A20162) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - PDE4A Rabbit pAb (A20162)

Immunofluorescence analysis of HeLa cells using PDE4A Rabbit pAb (A20162) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PDE4A Rabbit pAb (A20162)

Immunofluorescence analysis of K-562 cells using PDE4A Rabbit pAb (A20162) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PDE4A Rabbit pAb (A20162)

Immunofluorescence analysis of NIH/3T3 cells using PDE4A Rabbit pAb (A20162) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PDE4A Rabbit pAb (A20162)

Immunofluorescence analysis of PC-12 cells using PDE4A Rabbit pAb (A20162) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePDE4A Rabbit pAb
Catalog No.A20162
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 325-410 of human PDE4A (NP_001104777.1).
SequencePVPHLQPMSQITGLKKLMHSNSLNNSNIPRFGVKTDQEELLAQELENLNKWGLNIFCVSDYAGGRSLTCIMYMIFQERDLLKKFRI
Gene ID
Swiss Prot
SynonymsPDE4; DPDE2; PDE46; PDE4A
Calculated MW98kDa
Observed MW80kDa/110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, K-562, SH-SY5Y, Mouse testis, Mouse brain, Mouse heart, Rat testis, Rat brain
Cellular locationcytosol, nucleoplasm, nucleus, perinuclear region of cytoplasm, plasma membrane, ruffle membrane.
Customer validation

WB(Homo sapiens)

IF(Homo sapiens)

Documents

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    ABclonal:Western blot - PDE4A Rabbit pAb (A20162)}

    Western blot - PDE4A Rabbit pAb (A20162)

    Western blot analysis of various lysates using PDE4A Rabbit pAb (A20162) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.
    ABclonal:Immunohistochemistry - PDE4A Rabbit pAb (A20162)}

    Immunohistochemistry - PDE4A Rabbit pAb (A20162)

    Immunohistochemistry analysis of paraffin-embedded Rat kidney using PDE4A Rabbit pAb (A20162) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunofluorescence - PDE4A Rabbit pAb (A20162)}

    Immunofluorescence - PDE4A Rabbit pAb (A20162)

    Immunofluorescence analysis of HeLa cells using PDE4A Rabbit pAb (A20162) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - PDE4A Rabbit pAb (A20162)}

    Immunofluorescence - PDE4A Rabbit pAb (A20162)

    Immunofluorescence analysis of K-562 cells using PDE4A Rabbit pAb (A20162) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - PDE4A Rabbit pAb (A20162)}

    Immunofluorescence - PDE4A Rabbit pAb (A20162)

    Immunofluorescence analysis of NIH/3T3 cells using PDE4A Rabbit pAb (A20162) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - PDE4A Rabbit pAb (A20162)}

    Immunofluorescence - PDE4A Rabbit pAb (A20162)

    Immunofluorescence analysis of PC-12 cells using PDE4A Rabbit pAb (A20162) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20162? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)