제품 > 항체 > 단일클론항체(mAb)

PE Rabbit anti-Human CD28 mAb (A26814)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 Human PBMC were surface-stained with PE Rabbit anti-Human CD28 mAb (A26814, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Human PBMC were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human CD28 mAb (A26814, 5 μl/Test, right)

Overview

Product name	PE Rabbit anti-Human CD28 mAb
Catalog No.	A26814
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC5082-01-PE

Background

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant protein of human CD28 Full length (P10747).
Sequence	MLRLLLALNLFPSIQVTGNKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKPFWVLVVVGGVLACYSLLVTVAFIIFWVRSKRSRLLHSDYMNMTPRRPGPTRKHYQPYAPPRDFAAYRS
Gene ID
Swiss Prot
Synonyms	Tp44
Calculated MW	25kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	cell surface, cytosol, external side of plasma membrane, immunological synapse, plasma membrane, Membrane, Single-pass type I membrane protein, Cell surface.

Research Area

PE Rabbit anti-Human CD28 mAb images

Flow CytoMetry - PE Rabbit anti-Human CD28 mAb (A26814)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human CD28 mAb (A26814, 5 μl/Test, right)

* For research use only. Not for therapeutic or diagnostic purposes.