제품 > 항체 > 단일클론항체(mAb)

PE Rabbit anti-Human CD62E/E-Selectin mAb (A26630)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 HUVEC cells (untreated, left) and HUVEC cells (treated with 10ng/ml TNF-α, right) were surface-stained with PE Rabbit anti-Human CD62E/E-Selectin mAb (A26630, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 HUVEC cells (untreated, left) and HUVEC cells (treated with 10ng/ml TNF-α, right) were surface-stained with PE Rabbit anti-Human CD62E/E-Selectin mAb (A26630, 5 μl/Test).

Overview

Product name	PE Rabbit anti-Human CD62E/E-Selectin mAb
Catalog No.	A26630
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC61987-PE

Background

The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 22-230 of human CD62E/E-Selectin (NP_000441.2).
Sequence	WSYNTSTEAMTYDEASAYCQQRYTHLVAIQNKEEIEYLNSILSYSPSYYWIGIRKVNNVWVWVGTQKPLTEEAKNWAPGEPNNRQKDEDCVEIYIKREKDVGMWNDERCSKKKLALCYTAACTNTSCSGHGECVETINNYTCKCDPGFSGLKCEQIVNCTALESPEHGSLVCSHPLGNFSYNSSCSISCDRGYLPSSMETMQCMSSGEW
Gene ID
Swiss Prot
Synonyms	ELAM; ESEL; CD62E; ELAM1; LECAM2; selectin-e
Calculated MW	66kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Single-pass type I membrane protein.

Documents

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Research Area

PE Rabbit anti-Human CD62E/E-Selectin mAb images

Flow CytoMetry - PE Rabbit anti-Human CD62E/E-Selectin mAb (A26630)

Flow cytometry: 1X10^6 HUVEC cells (untreated, left) and HUVEC cells (treated with 10ng/ml TNF-α, right) were surface-stained with PE Rabbit anti-Human CD62E/E-Selectin mAb (A26630, 5 μl/Test).

* For research use only. Not for therapeutic or diagnostic purposes.