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PE Rabbit anti-Mouse CD19 mAb (A25265)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - PE Rabbit anti-Mouse CD19 mAb (A25265)

Flow cytometry: 1X10^6 RAW 264.7 cells (negative control, left) and A20 cells (right) were surface-stained with PE Rabbit anti-Mouse CD19 mAb (A25265, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - PE Rabbit anti-Mouse CD19 mAb (A25265)

Flow cytometry: 1X10^6 A20 cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Mouse CD19 mAb (A25265, 5 μl/Test, right).

Overview

Product namePE Rabbit anti-Mouse CD19 mAb
Catalog No.A25265
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5140-PE
Involved in several processes, including B-1 B cell differentiation; positive regulation of phosphatidylinositol 3-kinase activity; and positive regulation of release of sequestered calcium ion into cytosol. Acts upstream of or within B cell receptor signaling pathway. Located in external side of plasma membrane. Is integral component of plasma membrane. Is expressed in liver and spleen. Human ortholog(s) of this gene implicated in common variable immunodeficiency. Orthologous to human CD19 (CD19 molecule).
ImmunogenRecombinant protein of mouse CD19.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW60kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Single-pass type I membrane protein
    ABclonal:Flow CytoMetry - PE Rabbit anti-Mouse CD19 mAb (A25265)}

    Flow CytoMetry - PE Rabbit anti-Mouse CD19 mAb (A25265)

    Flow cytometry: 1X10^6 RAW 264.7 cells (negative control, left) and A20 cells (right) were surface-stained with PE Rabbit anti-Mouse CD19 mAb (A25265, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - PE Rabbit anti-Mouse CD19 mAb (A25265)}

    Flow CytoMetry - PE Rabbit anti-Mouse CD19 mAb (A25265)

    Flow cytometry: 1X10^6 A20 cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Mouse CD19 mAb (A25265, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

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