제품 > 항체 > 단일클론항체(mAb)

PerCP/Cyanine5.5 Rabbit anti-Mouse CD19 mAb (A27221)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry: 1X10^6 RAW 264.7 cells (negative control, left) and A20 cells (right) were surface-stained with PerCP/Cyanine5.5 Rabbit anti-Mouse CD19 mAb (A27221, 5 μl/Test, orange line) or PerCP/Cyanine5.5 Rabbit IgG isotype control (A25620, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 A20 cells were surface-stained with PerCP/Cyanine5.5 Rabbit IgG isotype control (A25620, 5 μl/Test, left) or PerCP/Cyanine5.5 Rabbit anti-Mouse CD19 mAb (A27221, 5 μl/Test, right).

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with PerCP/Cyanine5.5 Rabbit anti-Mouse CD19 mAb (A27221, 5 μl/Test, orange line) or PerCP/Cyanine5.5 Rabbit IgG isotype control (A25620, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line). Cells in the gate were used for analysis.

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with PerCP/Cyanine5.5 Rabbit IgG isotype control (A25620, 5 μl/Test, left) or PerCP/Cyanine5.5 Rabbit anti-Mouse CD19 mAb (A27221, 5 μl/Test, right).

Overview

Product name	PerCP/Cyanine5.5 Rabbit anti-Mouse CD19 mAb
Catalog No.	A27221
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC5140-01-PerCP-Cy5.5

Background

Involved in several processes, including B-1 B cell differentiation; positive regulation of phosphatidylinositol 3-kinase activity; and positive regulation of release of sequestered calcium ion into cytosol. Acts upstream of or within B cell receptor signaling pathway. Located in external side of plasma membrane. Is integral component of plasma membrane. Is expressed in liver and spleen. Human ortholog(s) of this gene implicated in common variable immunodeficiency. Orthologous to human CD19 (CD19 molecule).

Immunogen information

Immunogen	Recombinant protein of mouse CD19.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW	60kDa
Observed MW

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Single-pass type I membrane protein.