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PIAS1 Rabbit mAb (A4744)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - PIAS1 Rabbit mAb (A4744)

Western blot analysis of extracts of various cell lines, using PIAS1 Rabbit mAb (A4744) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - PIAS1 Rabbit mAb (A4744)

Western blot analysis of extracts of Mouse lung, using PIAS1 Rabbit mAb (A4744) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

Overview

Product namePIAS1 Rabbit mAb
Catalog No.A4744
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1116
This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 3-101 of human PIAS1 (O75925).
SequenceDSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYDG
Gene ID
Swiss Prot
SynonymsGBP; ZMIZ3; DDXBP1; GU/RH-II
Calculated MW72kDa
Observed MW76kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Hep G2, Jurkat, Mouse lung
Cellular locationNucleus, Nucleus speckle, PML body

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ABclonal:Western blot - PIAS1 Rabbit mAb (A4744)}

Western blot - PIAS1 Rabbit mAb (A4744)

Western blot analysis of extracts of various cell lines, using PIAS1 Rabbit mAb (A4744) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - PIAS1 Rabbit mAb (A4744)}

Western blot - PIAS1 Rabbit mAb (A4744)

Western blot analysis of extracts of Mouse lung, using PIAS1 Rabbit mAb (A4744) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

* For research use only. Not for therapeutic or diagnostic purposes.

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