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PITRM1 Rabbit pAb (A8362)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PITRM1 Rabbit pAb (A8362)

Western blot analysis of various lysates, using PITRM1 Rabbit pAb (A8362) at 1:6600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - PITRM1 Rabbit pAb (A8362)

Immunofluorescence analysis of L929 cells using PITRM1 Polyclonal Antibody (A8362) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product namePITRM1 Rabbit pAb
Catalog No.A8362
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is an ATP-dependent metalloprotease that degrades post-cleavage mitochondrial transit peptides. The encoded protein binds zinc and can also degrade amyloid beta A4 protein, suggesting a possible role in Alzheimer's disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 758-1037 of human PITRM1 (NP_055704.2).
SequenceIKPILRKLPRIKKHLLNGDNMRCSVNATPQQMPQTEKAVEDFLRSIGRSKKERRPVRPHTVEKPVPSSSGGDAHVPHGSQVIRKLVMEPTFKPWQMKTHFLMPFPVNYVGECIRTVPYTDPDHASLKILARLMTAKFLHTEIREKGGAYGGGAKLSHNGIFTLYSYRDPNTIETLQSFGKAVDWAKSGKFTQQDIDEAKLSVFSTVDAPVAPSDKGMDHFLYGLSDEMKQAHREQLFAVSHDKLLAVSDRYLGTGKSTHGLAILGPENPKIAKDPSWIIQ
Gene ID
Swiss Prot
SynonymsMP1; PreP; SCAR30
Calculated MW117kDa
Observed MW117kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-87MG, NTERA-2
Cellular locationMitochondrion matrix

Documents

Certificate of Compliance

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    ABclonal:Western blot - PITRM1 Rabbit pAb (A8362)}

    Western blot - PITRM1 Rabbit pAb (A8362)

    Western blot analysis of various lysates, using PITRM1 Rabbit pAb (A8362) at 1:6600 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - PITRM1 Rabbit pAb (A8362)}

    Immunofluorescence - PITRM1 Rabbit pAb (A8362)

    Immunofluorescence analysis of L929 cells using PITRM1 Polyclonal Antibody (A8362) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)