제품 > 항체 > 다중클론항체(pAb)

PP1 beta Rabbit pAb (A13529)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - PP1 beta Rabbit pAb (A13529)

Western blot analysis of extracts of SW480 cells, using PP1 beta antibody (A13529) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - PP1 beta Rabbit pAb (A13529)

Immunofluorescence analysis of U2OS cells using PP1 beta antibody (A13529). Blue: DAPI for nuclear staining.

Overview

Product namePP1 beta Rabbit pAb
Catalog No.A13529
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human PP1 beta (NP_002700.1).
SequenceMADGELNVDSLITRLLEVRGCRPGKIVQMTEAEVRGLCIKSREIFLSQPILLELEAPLKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR
Gene ID
Swiss Prot
SynonymsMP; PP1B; PP1c; NSLH2; PP-1B; PPP1CD; PP1beta; PPP1beta; HEL-S-80p
Calculated MW37kDa
Observed MW37kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSW480
Cellular locationCytoplasm, Nucleus, nucleolus, nucleoplasm

Documents

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ABclonal:Western blot - PP1 beta Rabbit pAb (A13529)}

Western blot - PP1 beta Rabbit pAb (A13529)

Western blot analysis of extracts of SW480 cells, using PP1 beta antibody (A13529) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - PP1 beta Rabbit pAb (A13529)}

Immunofluorescence - PP1 beta Rabbit pAb (A13529)

Immunofluorescence analysis of U2OS cells using PP1 beta antibody (A13529). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

Secondary Antibodies (26)