제품 > 항체 > 다중클론항체(pAb)

PREX1 Rabbit pAb (A18344)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - PREX1 Rabbit pAb (A18344)

Western blot analysis of lysates from T-47D cells using PREX1 Rabbit pAb (A18344) at 1:3700 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 60 s.

Overview

Product namePREX1 Rabbit pAb
Catalog No.A18344
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3, 4, 5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1000-1300 of human PREX1 (NP_065871.2).
SequencePSLIGLDPEQGHLNPMSYTQHCITTMAAPSWKCLPAAEGDPQGQGLHDGSFGPASGTLGQEDRGLSFLLKQEDREIQDAYLQLFTKLDVALKEMKQYVTQINRLLSTITEPTSGGSCDASLAEEASSLPLVSEESEMDRSDHGGIKKVCFKVAEEDQEDSGHDTMSYRDSYSECNSNRDSVLSYTSVRSNSSYLGSDEMGSGDELPCDMRIPSDKQDKLHGCLEHLFNQVDSINALLKGPVMSRAFEETKHFPMNHSLQEFKQKEECTIRGRSLIQISIQEDPWNLPNSIKTLVDNIQRYV
Gene ID
Swiss Prot
SynonymsP-REX1; PREX1
Calculated MW186kDa
Observed MW190kDa/
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesT-47D,
Cellular locationcytosol, plasma membrane.

Documents

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    ABclonal:Western blot - PREX1 Rabbit pAb (A18344)}

    Western blot - PREX1 Rabbit pAb (A18344)

    Western blot analysis of lysates from T-47D cells using PREX1 Rabbit pAb (A18344) at 1:3700 dilution incubated overnight at 4°C.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020)
    Exposure time: 60 s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)