제품 > 항체 > 단일클론항체(mAb)

PRKAR2B Rabbit mAb (A19745)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - PRKAR2B Rabbit mAb (A19745)

Western blot analysis of various lysates using PRKAR2B Rabbit mAb (A19745) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - PRKAR2B Rabbit mAb (A19745)

Immunohistochemistry analysis of paraffin-embedded mouse brain using PRKAR2B Rabbit mAb (A19745) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PRKAR2B Rabbit mAb (A19745)

Immunohistochemistry analysis of paraffin-embedded rat ovary using PRKAR2B Rabbit mAb (A19745) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product namePRKAR2B Rabbit mAb
Catalog No.A19745
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2272
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 319-418 of human PRKAR2B (P31323).
SequenceGEVKITMKRKGKSEVEENGAVEIARCSRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA
Gene ID
Swiss Prot
SynonymsPRKAR2; RII-BETA; PRKAR2B
Calculated MW46kDa
Observed MW46kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse brian, Rat brain
Cellular locationCytoplasm, Cell membrane

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ABclonal:Western blot - PRKAR2B Rabbit mAb (A19745)}

Western blot - PRKAR2B Rabbit mAb (A19745)

Western blot analysis of various lysates using PRKAR2B Rabbit mAb (A19745) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - PRKAR2B Rabbit mAb (A19745)}

Immunohistochemistry - PRKAR2B Rabbit mAb (A19745)

Immunohistochemistry analysis of paraffin-embedded mouse brain using PRKAR2B Rabbit mAb (A19745) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PRKAR2B Rabbit mAb (A19745)}

Immunohistochemistry - PRKAR2B Rabbit mAb (A19745)

Immunohistochemistry analysis of paraffin-embedded rat ovary using PRKAR2B Rabbit mAb (A19745) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)