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PSD93/chapsyn-110/DLG2 Rabbit mAb (A19669)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PSD93/chapsyn-110/DLG2 Rabbit mAb (A19669)

Western blot analysis of extracts of various cell lines, using PSD93/chapsyn-110/DLG2 Rabbit mAb (A19669) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - PSD93/chapsyn-110/DLG2 Rabbit mAb (A19669)

Immunofluorescence analysis of mouse brain cells using PSD93/chapsyn-110/DLG2 Rabbit mAb (A19669) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product namePSD93/chapsyn-110/DLG2 Rabbit mAb
Catalog No.A19669
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2222
This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. The encoded protein forms a heterodimer with a related family member that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described, but their full-length nature is not known.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 600-700 of human PSD93/chapsyn-110/DLG2 (Q15700).
SequenceRRVERKERARLKTVKFNAKPGVIDSKGSFNDKRKKSFIFSRKFPFYKNKEQSEQETSDPERGQEDLILSYEPVTRQEINYTRPVIILGPMKDRINDDLISE
Gene ID
Swiss Prot
SynonymsPSD93; PSD-93; PPP1R58; chapsyn-110
Calculated MW98kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, C6, SH-SY5Y, U-251MG, Mouse lung, Mouse spinal cord, Mouse brain, Rat brain
Cellular locationAxon cytoplasm, basolateral plasma membrane, cytosol, neuromuscular junction, neuron projection, perikaryon, plasma membrane, postsynaptic density, postsynaptic density membrane
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - PSD93/chapsyn-110/DLG2 Rabbit mAb (A19669)}

Western blot - PSD93/chapsyn-110/DLG2 Rabbit mAb (A19669)

Western blot analysis of extracts of various cell lines, using PSD93/chapsyn-110/DLG2 Rabbit mAb (A19669) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - PSD93/chapsyn-110/DLG2 Rabbit mAb (A19669)}

Immunofluorescence - PSD93/chapsyn-110/DLG2 Rabbit mAb (A19669)

Immunofluorescence analysis of mouse brain cells using PSD93/chapsyn-110/DLG2 Rabbit mAb (A19669) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A19669? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)