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Phospho-PSD93/chapsyn-110/DLG2-Y340 Rabbit pAb (AP1080)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-PSD93/chapsyn-110/DLG2-Y340 Rabbit pAb (AP1080) at 1:1000 dilution. 293T cells were treated by ATP(5 mM) at 30℃ for 1 hour. NIH/3T3 cells were treated by ATP(5 mM) at 30℃ for 1 hour. C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	Phospho-PSD93/chapsyn-110/DLG2-Y340 Rabbit pAb
Catalog No.	AP1080
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. The encoded protein forms a heterodimer with a related family member that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described, but their full-length nature is not known.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y340 of human PSD93/chapsyn-110/DLG2 (NP_001355.2).
Sequence	DDYTR
Gene ID
Swiss Prot
Synonyms	PSD93; PSD-93; PPP1R58; chapsyn-110; Phospho-PSD93/chapsyn-110/DLG2-Y340
Calculated MW	98kDa
Observed MW	105kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T treated by ATP, NIH/3T3 treated by ATP, C6 treated by ATP
Cellular location	axon cytoplasm, basolateral plasma membrane, cytosol, neuromuscular junction, neuron projection, perikaryon, plasma membrane, postsynaptic density, postsynaptic density membrane.