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PTP1B Rabbit mAb (A23035)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - PTP1B Rabbit mAb (A23035)

Western blot analysis of various lysates, using PTP1B Rabbit mAb (A23035) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - PTP1B Rabbit mAb (A23035)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PTP1B Rabbit mAb (A23035) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PTP1B Rabbit mAb (A23035)

Immunohistochemistry analysis of paraffin-embedded Mouse colon using PTP1B Rabbit mAb (A23035) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product namePTP1B Rabbit mAb
Catalog No.A23035
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC58516
The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 281-435 of human PTP1B (NP_002818.1).
SequenceIMGDSSVQDQWKELSHEDLEPPPEHIPPPPRPPKRILEPHNGKCREFFPNHQWVKEETQEDKDCPIKEEKGSPLNAAPYGIESMSQDTEVRSRVVGGSLRGAQAASPAKGEPSLPEKDEDHALSYWKPFLVNMCVATVLTAGAYLCYRFLFNSNT
Gene ID
Swiss Prot
SynonymsPTP1B
Calculated MW50kDa
Observed MW50kDa/48kDa/46kDa/37-39kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesJurkat, HeLa
Cellular locationcytoplasm, cytosol, early endosome, endoplasmic reticulum, endosome lumen, mitochondrial crista, mitochondrial matrix, sorting endosome

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ABclonal:Western blot - PTP1B Rabbit mAb (A23035)}

Western blot - PTP1B Rabbit mAb (A23035)

Western blot analysis of various lysates, using PTP1B Rabbit mAb (A23035) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - PTP1B Rabbit mAb (A23035)}

Immunohistochemistry - PTP1B Rabbit mAb (A23035)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PTP1B Rabbit mAb (A23035) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PTP1B Rabbit mAb (A23035)}

Immunohistochemistry - PTP1B Rabbit mAb (A23035)

Immunohistochemistry analysis of paraffin-embedded Mouse colon using PTP1B Rabbit mAb (A23035) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)