제품 > 항체 > 다중클론항체(pAb)

PTPN1 Rabbit pAb (A1590)

Reviews (2)Publications (7) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PTPN1 Rabbit pAb (A1590)

Western blot analysis of extracts of various cell lines, using PTPN1 antibody (A1590) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - PTPN1 Rabbit pAb (A1590)

Immunofluorescence analysis of U2OS cells using PTPN1 Rabbit pAb (A1590) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for PTPN1 Rabbit pAb(A1590)
Review
ABclonal: review for PTPN1 Rabbit pAb(A1590)

Overview

Product namePTPN1 Rabbit pAb
Catalog No.A1590
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 281-435 of human PTPN1 (NP_002818.1).
SequenceIMGDSSVQDQWKELSHEDLEPPPEHIPPPPRPPKRILEPHNGKCREFFPNHQWVKEETQEDKDCPIKEEKGSPLNAAPYGIESMSQDTEVRSRVVGGSLRGAQAASPAKGEPSLPEKDEDHALSYWKPFLVNMCVATVLTAGAYLCYRFLFNSNT
Gene ID
Swiss Prot
SynonymsPTP1B
Calculated MW50kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesBT-474, SKOV3, U-251MG, Mouse brain, Rat brain
Cellular locationCytoplasmic side, Endoplasmic reticulum membrane, Peripheral membrane protein
Customer validation

(Mouse liver, A549)

WB(Mus musculus, Homo sapiens)

IF(Mus musculus)

IHC(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - PTPN1 Rabbit pAb (A1590)}

Western blot - PTPN1 Rabbit pAb (A1590)

Western blot analysis of extracts of various cell lines, using PTPN1 antibody (A1590) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - PTPN1 Rabbit pAb (A1590)}

Immunofluorescence - PTPN1 Rabbit pAb (A1590)

Immunofluorescence analysis of U2OS cells using PTPN1 Rabbit pAb (A1590) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A1590

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Publishing research using A1590? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)