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Parvalbumin/PVALB Rabbit pAb (A13538)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Parvalbumin/PVALB Rabbit pAb (A13538)

Western blot analysis of various lysates using Parvalbumin (PVALB) Rabbit pAb (A13538) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - Parvalbumin/PVALB Rabbit pAb (A13538)

Western blot analysis of various lysates using Parvalbumin (PVALB) Rabbit pAb (A13538) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Parvalbumin/PVALB Rabbit pAb (A13538)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using Parvalbumin (PVALB) Rabbit pAb (A13538) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - Parvalbumin/PVALB Rabbit pAb (A13538)

Immunofluorescence analysis of paraffin-embedded Rat cerebellum tissue using Parvalbumin (PVALB) Rabbit pAb(A13538) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameParvalbumin/PVALB Rabbit pAb
Catalog No.A13538
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human Parvalbumin (Parvalbumin (PVALB)) (NP_002845.1).
SequenceMSMTDLLNAEDIKKAVGAFSATDSFDHKKFFQMVGLKKKSADDVKKVFHMLDKDKSGFIEEDELGFILKGFSPDARDLSAKETKMLMAAGDKDGDGKIGVDEFSTLVAES
Gene ID
Swiss Prot
SynonymsD22S749; Parvalbumin (PVALB)
Calculated MW12kDa
Observed MW15kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Mouse skeletal muscle, Rat brain, Rat skeletal muscle
Cellular locationaxon, cytoplasm, nucleus, synapse.
Customer validation

IF(Mus musculus, Homo sapiens)

WB(Rattus norvegicus, Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - Parvalbumin/PVALB Rabbit pAb (A13538)}

Western blot - Parvalbumin/PVALB Rabbit pAb (A13538)

Western blot analysis of various lysates using Parvalbumin (PVALB) Rabbit pAb (A13538) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - Parvalbumin/PVALB Rabbit pAb (A13538)}

Western blot - Parvalbumin/PVALB Rabbit pAb (A13538)

Western blot analysis of various lysates using Parvalbumin (PVALB) Rabbit pAb (A13538) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - Parvalbumin/PVALB Rabbit pAb (A13538)}

Immunohistochemistry - Parvalbumin/PVALB Rabbit pAb (A13538)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using Parvalbumin (PVALB) Rabbit pAb (A13538) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - Parvalbumin/PVALB Rabbit pAb (A13538)}

Immunofluorescence - Parvalbumin/PVALB Rabbit pAb (A13538)

Immunofluorescence analysis of paraffin-embedded Rat cerebellum tissue using Parvalbumin (PVALB) Rabbit pAb(A13538) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A13538? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

ELISA 키트 (1)

Secondary Antibodies (26)