Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of lysates from HeLa cells, using Phospho-AKT1S1/PRAS40-T246 Rabbit pAb (AP0793) at 1:400 dilution. HeLa cells were treated by Insulin (100 nM) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 45s.
| Product name | Phospho-AKT1S1/PRAS40-T246 Rabbit pAb |
|---|---|
| Catalog No. | AP0793 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around T246 of human AKT1S1/PRAS40 (NP_001092102.1). |
|---|---|
| Sequence | LNTSD |
| Gene ID | |
| Swiss Prot | |
| Synonyms | Lobe; PRAS40; Phospho-AKT1S1/PRAS40-T246 |
| Calculated MW | 27kDa |
| Observed MW | 40kDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HeLa treated by Insulin |
| Cellular location | Cytoplasm, cytosol. |
| Customer validation | (293T 293TStarvation 293T FBS 293T Insulin 293T EGF HelaStarvation Hela FBS Hela Insulin Hela EGF ) |
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