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Phospho-Cdk1/2-T14 Rabbit mAb (AP1001)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-Cdk1/2-T14 Rabbit mAb (AP1001) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated by Nocodazole (100 ng/mL) at 37℃ for 24 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name	Phospho-Cdk1/2-T14 Rabbit mAb
Catalog No.	AP1001
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1546

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T14 of human Cdk1/2CDK1 (P06493).
Sequence	EGTYG
Gene ID
Swiss Prot
Synonyms	CDC2; CDC28A; P34CDC2; Phospho-Cdk1/2-T14
Calculated MW	34kDa
Observed MW	34kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by Hydroxyurea, HeLa treated by Nocodazole, NIH/3T3 treated by Hydroxyurea, NIH/3T3 treated by Nocodazole, C6 treated by Hydroxyurea, C6 treated by Nocodazole
Cellular location	Centrosome, Cytoplasm, Cytosol, Extracellular exosome, Mitochondrial matrix, Mitochondrion, Mitotic spindle, Nucleoplasm, Nucleus, Spindle microtubule.