제품 > 항체 > 인산화 특이적 항체

Phospho-DRP1-S616 Rabbit mAb (AP1353)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates, using Phospho-DRP1-S616 Rabbit mAb (AP1353) at 1:10000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:100000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product name	Phospho-DRP1-S616 Rabbit mAb
Catalog No.	AP1353
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC55743

Background

This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S616 of human DNM1L (NP_036192.2).
Sequence	PASPQ
Gene ID
Swiss Prot
Synonyms	DLP1; DRP1; DVLP; EMPF; OPA5; EMPF1; DYMPLE; HDYNIV; Phospho-DRP1-S616
Calculated MW	82kDa
Observed MW	78-82kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by Calyculin A
Cellular location	Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Golgi apparatus, Membrane, Mitochondrion outer membrane, Peripheral membrane protein, Peroxisome, clathrin-coated pit, cytosol, secretory vesicle, synaptic vesicle membrane.
Customer validation	WB(Homo sapiens, Mus musculus) IF(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-DRP1-S616 Rabbit mAb images

Western blot - Phospho-DRP1-S616 Rabbit mAb (AP1353)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-DRP1-S616 Rabbit mAb (AP1353)

Product:

Phospho-DRP1-S616 Rabbit mAb

Journal:

Cell biology international

Application:

IF:

3.9

Species:

Homo sapiens

PMID:

35819094

Title:

Tamoxifen modulates mitochondrial dynamics through AMPK and MAPK during nutrition deprivation

References for Phospho-DRP1-S616 Rabbit mAb (AP1353)

Product:

Phospho-DRP1-S616 Rabbit mAb

Journal:

Biochemical and biophysical research communications

Application:

IF:

2.5

Species:

Homo sapiens

PMID:

36584588

Title:

GPER induces mitochondrial fission through p44/42 MAPK-Drp1 pathway in breast cancer cells

References for Phospho-DRP1-S616 Rabbit mAb (AP1353)

Product:

Phospho-DRP1-S616 Rabbit mAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

36461586

Title:

AMPK/Drp1 Pathway Mediates Streptococcus Uberis-Induced Mitochondrial Dysfunction

References for Phospho-DRP1-S616 Rabbit mAb (AP1353)

Product:

Phospho-DRP1-S616 Rabbit mAb

Journal:

Journal of cellular and molecular medicine

Application:

IF:

5.3

Species:

Homo sapiens

PMID:

37278388

Title:

RhoA/ROCK1 regulates the mitochondrial dysfunction through Drp1 induced by Porphyromonas gingivalis in endothelial cells - PMC

References for Phospho-DRP1-S616 Rabbit mAb (AP1353)

Product:

Phospho-DRP1-S616 Rabbit mAb

Journal:

Oncogene

Application:

IF:

6.9

Species:

Homo sapiens

PMID:

36759571

Title:

Retrograde regulation of mitochondrial fission and epithelial to mesenchymal transition in hepatocellular carcinoma by GCN5L1

References for Phospho-DRP1-S616 Rabbit mAb (AP1353)

Product:

Phospho-DRP1-S616 Rabbit mAb

Journal:

BioFactors (Oxford, England)

Application:

IF:

Species:

Mus musculus

PMID:

37596888

Title:

Dapagliflozin improves diabetic cognitive impairment via indirectly modulating the mitochondria homeostasis of hippocampus in diabetic mice

References for Phospho-DRP1-S616 Rabbit mAb (AP1353)

Product:

Phospho-DRP1-S616 Rabbit mAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

6.9

Species:

Homo sapiens

PMID:

37633053

Title:

Inhibition of mitochondrial fusion via SIRT1/PDK2/PARL axis breaks mitochondrial metabolic plasticity and sensitizes cancer cells to glucose restriction

References for Phospho-DRP1-S616 Rabbit mAb (AP1353)

Product:

Phospho-DRP1-S616 Rabbit mAb

Journal:

Oxidative medicine and cellular longevity

Application:

WB, IF

IF:

Species:

Mus musculus

PMID:

35958024