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DRP1 Rabbit pAb (A2586)

Reviews (3)Publications (55) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DRP1 Rabbit pAb (A2586)

Western blot analysis of various lysates using DRP1 Rabbit pAb (A2586) at 1:8000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - DRP1 Rabbit pAb (A2586)

Immunohistochemistry analysis of paraffin-embedded Rat brain using DRP1 Rabbit pAb (A2586) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - DRP1 Rabbit pAb (A2586)

Immunohistochemistry analysis of paraffin-embedded Rat heart using DRP1 Rabbit pAb (A2586) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - DRP1 Rabbit pAb (A2586)

Immunofluorescence analysis of NIH/3T3 cells using DRP1 Rabbit pAb (A2586) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for DRP1 Rabbit pAb(A2586)
Review
ABclonal: review for DRP1 Rabbit pAb(A2586)
Review
ABclonal: review for DRP1 Rabbit pAb(A2586)

Overview

Product nameDRP1 Rabbit pAb
Catalog No.A2586
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 411-710 of human DRP1 (NP_036193.2).
SequenceVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKLIQDSRRETKNVASGGGGVGDGVQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW
Gene ID
Swiss Prot
SynonymsDLP1; DRP1; DVLP; EMPF; OPA5; EMPF1; DYMPLE; HDYNIV
Calculated MW82kDa
Observed MW78kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Rat brain
Cellular locationCytoplasm, Cytoplasmic vesicle, Endomembrane system, Golgi apparatus, Membrane, Mitochondrion outer membrane, Peripheral membrane protein, Peroxisome, clathrin-coated pit, cytosol, secretory vesicle, synaptic vesicle membrane
Customer validation

(Human bone marrow cancer cell, HeLa, Mouse skeletal muscle)

WB(Rattus norvegicus, Homo sapiens, Mus musculus, Gallus gallus, Yellow catfish, Ctenopharyngodon idellus)

IF(Homo sapiens, Mus musculus)

Co-IP(Homo sapiens)

IF(Rattus norvegicus, Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - DRP1 Rabbit pAb (A2586)}

Western blot - DRP1 Rabbit pAb (A2586)

Western blot analysis of various lysates using DRP1 Rabbit pAb (A2586) at 1:8000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - DRP1 Rabbit pAb (A2586)}

Immunohistochemistry - DRP1 Rabbit pAb (A2586)

Immunohistochemistry analysis of paraffin-embedded Rat brain using DRP1 Rabbit pAb (A2586) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - DRP1 Rabbit pAb (A2586)}

Immunohistochemistry - DRP1 Rabbit pAb (A2586)

Immunohistochemistry analysis of paraffin-embedded Rat heart using DRP1 Rabbit pAb (A2586) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - DRP1 Rabbit pAb (A2586)}

Immunofluorescence - DRP1 Rabbit pAb (A2586)

Immunofluorescence analysis of NIH/3T3 cells using DRP1 Rabbit pAb (A2586) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (4)

Secondary Antibodies (26)