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Phospho-FOXO3A-S294 Rabbit pAb (AP0856)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-FOXO3A-S294 Rabbit pAb (AP0856) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	Phospho-FOXO3A-S294 Rabbit pAb
Catalog No.	AP0856
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S294 of human FOXO3A (NP_001446.1).
Sequence	PGSPT
Gene ID
Swiss Prot
Synonyms	FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2; Phospho-FOXO3A-S294
Calculated MW	71kDa
Observed MW	82kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3 treated by FBS
Cellular location	Cytoplasm, Nucleus, cytosol.
Customer validation	WB(Rattus norvegicus, Mus musculus, Rattus norvegicus)