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Phospho-FOXO3A-S425 Rabbit pAb (AP1200)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from K-562 cells, using Phospho-FOXO3A-S425 Rabbit pAb (AP1200) at 1:1000 dilution. K-562 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Phospho-FOXO3A-S425 Rabbit pAb (AP1200)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-FOXO3A-S425 Rabbit pAb (AP1200) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Phospho-FOXO3A-S425 Rabbit pAb (AP1200) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse heart using Phospho-FOXO3A-S425 Rabbit pAb (AP1200) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	Phospho-FOXO3A-S425 Rabbit pAb
Catalog No.	AP1200
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S425 of human FOXO3A (NP_001446.1).
Sequence	LGSPT
Gene ID
Swiss Prot
Synonyms	FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2; Phospho-FOXO3A-S425
Calculated MW	71kDa
Observed MW	82kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	K-562 treated by FBS
Cellular location	cytoplasm, cytosol, mitochondrial matrix, mitochondrial outer membrane, nucleoplasm, nucleus.