Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using Phospho-GYS1-S641 Rabbit pAb (AP0877) at 1:1000 dilution.NIH/3T3 cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of lysates from 293T cells, using Phospho-GYS1-S641 pAb (AP0877) at 1:1000 dilution or Glycogen synthase 1 (GYS1) antibody (A2519).293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
| Product name | Phospho-GYS1-S641 Rabbit pAb |
|---|---|
| Catalog No. | AP0877 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around S641 of human Glycogen synthase 1 (GYS1) (NP_002094.2). |
|---|---|
| Sequence | PASVP |
| Gene ID | |
| Swiss Prot | |
| Synonyms | GSY; GYS; Phospho-GYS1-S641 |
| Calculated MW | 84kDa |
| Observed MW | 100kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | 293T, NIH/3T3, C6 |
| Cellular location | cytoplasm, cytosol |
| Customer validation | WB(Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using AP0877? Please let us know so that we can cite the reference in this datasheet.
_WB_01.jpg?t=1709544514 "ABclonal:Western blot - Phospho-GYS1-S641 Rabbit pAb (AP0877)")
_WB_02.jpg?t=1709544514 "ABclonal:Western blot - Phospho-GYS1-S641 Rabbit pAb (AP0877)")
