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Phospho-Glucocorticoid Receptor-S211 Rabbit pAb (AP0759)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Phospho-Glucocorticoid Receptor-S211 Rabbit pAb (AP0759)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Glucocorticoid Receptor-S211 Rabbit pAb (AP0759) at 1:600 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product namePhospho-Glucocorticoid Receptor-S211 Rabbit pAb
Catalog No.AP0759
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175).
ImmunogenA synthetic phosphorylated peptide around S211 of human Glucocorticoid Receptor (NP_000167.1).
SequenceNESPW
Gene ID
Swiss Prot
SynonymsGR; GCR; GRL; GCCR; GCRST; Phospho-Glucocorticoid Receptor-S211
Calculated MW86kDa
Observed MW95kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesNIH/3T3 treated by Calyculin A
Cellular locationCytoplasm, Mitochondrion, Nucleus, Nucleus.
Customer validation

WB(Mus musculus)

Documents

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ABclonal:Western blot - Phospho-Glucocorticoid Receptor-S211 Rabbit pAb (AP0759)}

Western blot - Phospho-Glucocorticoid Receptor-S211 Rabbit pAb (AP0759)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Glucocorticoid Receptor-S211 Rabbit pAb (AP0759) at 1:600 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)