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Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit mAb (AP1394)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from 293T cells, using Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit mAb (AP1394) at 1:1000 dilution. 293T cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit mAb (AP1394)

Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit mAb (AP1394) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit mAb (AP1394) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit mAb
Catalog No.	AP1394
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC56536

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]

Immunogen information

Immunogen	A phospho synthetic peptide corresponding to residues surrounding S246&S259&S220 of Human HDAC4&HDAC5-S259&HDAC9.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	HDAC4/HDAC5/HDAC9; Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220
Calculated MW	57-65kDa/97-122kDa
Observed MW	124kDa/140kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	293T treated by EGF
Cellular location	cytoplasm, cytosol, histone deacetylase complex, nuclear speck, nucleoplasm, nucleus