제품 > 항체 > 인산화 특이적 항체

Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb (AP0280)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from 293 cells, using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb (AP0280). 293 cells were treated by EGF.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

ABclonal:Immunohistochemistry - Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb (AP0280)

Immunohistochemistry analysis of paraffin-embedded Human placenta using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb (AP0280) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb (AP0280) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb (AP0280) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb
Catalog No.	AP0280
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]

Immunogen information

Immunogen	A phospho specific peptide corresponding to residues surrounding S246 of human HDAC4
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	HDAC4/HDAC5/HDAC9; Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220
Calculated MW	57-65kDa/97-122kDa
Observed MW	125kDa/140kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	293 treated by EGF
Cellular location	cytoplasm, cytosol, histone deacetylase complex, nuclear speck, nucleoplasm, nucleus.