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Phospho-IRAK4-Ser346 Rabbit mAb (AP1457)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from Karpas 299 cells using Phospho-IRAK4-Ser346 Rabbit mAb (AP1457) at 1:1000 dilution. Karpas 299 cells were treated by IL-1β (200 ng/ml) at 37℃ for 15 minutes after serum starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 75s.

Overview

Product name	Phospho-IRAK4-Ser346 Rabbit mAb
Catalog No.	AP1457
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC64221

Background

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Ser346 of human IRAK4 (NP_057207.2).
Sequence	VMTSRI
Gene ID
Swiss Prot
Synonyms	IPD1; IMD67; REN64; IRAK-4; NY-REN-64
Calculated MW	52kDa
Observed MW	57kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Karpas 299 treated by IL-1β
Cellular location	Cytoplasm