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Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)

Western blot analysis of lysates from HeLa cells, using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at 1:1000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)

Immunohistochemistry analysis of paraffin-embedded Human breast using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)

Immunohistochemistry analysis of paraffin-embedded Mouse colon using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)

Immunohistochemistry analysis of paraffin-embedded Rat colon using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)

Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product namePhospho-MKK3-S189+MKK6-S207 Rabbit mAb
Catalog No.AP1449
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC62482
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]
ImmunogenA synthetic phosphorylated peptide around S207 of human MKK3/MKK6(P46734/P52564).
SequenceVDSVA
Gene ID
Swiss Prot
SynonymsMAP2K3/MAP2K6; Phospho-MKK3-S189+MKK6-S207
Calculated MW36kDa/39kDa/31kDa/37kDa
Observed MW38kDa/40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa treated by UV, NIH/3T3 treated by UV
Cellular locationcytosol, nucleoplasm

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)}

    Western blot - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)

    Western blot analysis of lysates from HeLa cells, using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at 1:1000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Western blot - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)}

    Western blot - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)

    Western blot analysis of lysates from NIH/3T3 cells, using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)}

    Immunohistochemistry - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)

    Immunohistochemistry analysis of paraffin-embedded Human breast using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)}

    Immunohistochemistry - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)

    Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)}

    Immunohistochemistry - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)

    Immunohistochemistry analysis of paraffin-embedded Mouse colon using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)}

    Immunohistochemistry - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)

    Immunohistochemistry analysis of paraffin-embedded Rat colon using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)}

    Immunohistochemistry - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449)

    Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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