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Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1512)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from HeLa cells, using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1512) at 1:1000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1512) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1512)

Immunofluorescence analysis of HeLa cells (treated with UV) and HeLa cells (untreated) using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb(AP1512) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Phospho-MKK3-S189+MKK6-S207 Rabbit mAb
Catalog No.	AP1512
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC62483

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S189/S207 of human MKK3/MKK6.
Sequence	VDSVA
Gene ID
Swiss Prot
Synonyms	MAP2K3/MAP2K6; Phospho-MKK3-S189+MKK6-S207
Calculated MW	36kDa/39kDa/31kDa/37kDa
Observed MW	38kDa/40kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa treated by UV, NIH/3T3 treated by UV
Cellular location	cytosol, nucleoplasm