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Phospho-PAK1/2/3-S144/S141/S139 Rabbit mAb (AP1158)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-PAK1/2/3-S144/S141/S139 Rabbit mAb (AP1158) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	Phospho-PAK1/2/3-S144/S141/S139 Rabbit mAb
Catalog No.	AP1158
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1698

Background

This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S144 of human PAK1PAK1 (Q13153).
Sequence	YMSFT
Gene ID
Swiss Prot
Synonyms	PAKalpha; Phospho-PAK1/2/3-S144/S141/S139
Calculated MW	65kDa
Observed MW	65kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3 treated by Forskolin
Cellular location	cytoplasm, cytosol, focal adhesion, microtubule organizing center, nuclear membrane, nucleoplasm, plasma membrane, ruffle membrane, Z disc.
Customer validation	WB(Homo sapiens,Rattus norvegicus)