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Phospho-PI3 Kinase p55-Y199 Rabbit mAb (AP1463)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of lysates from C2C12 cells using Phospho-PI3 Kinase p55-Y199 Rabbit mAb (AP1463) at 1:5000 dilution. C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - Phospho-PI3 Kinase p55-Y199 Rabbit mAb (AP1463)

Confocal imaging of C2C12 cells (treated with H2O2) and C2C12 (untreated) cells using Phospho-PI3 Kinase p55-Y199 Rabbit mAb (AP1463, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name	Phospho-PI3 Kinase p55-Y199 Rabbit mAb
Catalog No.	AP1463
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC65199

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y199 of human PI3 Kinase p55（NP_001107644.1）.
Sequence	RLYEEY
Gene ID
Swiss Prot
Synonyms	PIK3R3; Phospho-PI3KP55γ-Y199
Calculated MW	55kDa
Observed MW	62kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:6000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	C2C12 treated by Hydrogen Peroxide
Cellular location	cytosol.