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Phospho-PKC delta-S645 Rabbit pAb (AP0972)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from 293T cells, using Phospho-PKC delta-S645 pAb (AP0972) at 1:1000 dilution or PKC delta antibody (A0471). 293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	Phospho-PKC delta-S645 Rabbit pAb
Catalog No.	AP0972
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S645 of human PKC delta (NP_006245.2).
Sequence	RLSYS
Gene ID
Swiss Prot
Synonyms	MAY1; PKCD; ALPS3; CVID9; nPKC-delta; Phospho-PKC delta-S645
Calculated MW	78kDa
Observed MW	80kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T treated by PMA
Cellular location	Cell membrane, Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus, Peripheral membrane protein, perinuclear region.
Customer validation	WB(Rattus norvegicus)