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Phospho-PKC delta-Y311 Rabbit pAb (AP0496)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from U937 and Raw264.7 cells, using Phospho-PKC delta-Y311 Rabbit pAb (AP0496) at 1:1000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes.RAW 264.7 cells were treated by PMA (200 nM) for 30 minutes .
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name	Phospho-PKC delta-Y311 Rabbit pAb
Catalog No.	AP0496
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y311 of rat PKC delta (NP_579841.1).
Sequence	VGIYQ
Gene ID
Swiss Prot
Synonyms	MAY1; PKCD; ALPS3; CVID9; nPKC-delta; Phospho-PKC delta-Y311
Calculated MW	78kDa
Observed MW	78kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	U-937 treated by PMA, RAW 264.7 treated by PMA
Cellular location	Cell membrane, Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus, Peripheral membrane protein, perinuclear region.