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Phospho-RPA2-S4/S8 Rabbit pAb (AP1102)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Rat

ABclonal:Western blot - Phospho-RPA2-S4/S8 Rabbit pAb (AP1102)

Western blot analysis of lysates from C6 cells, using Phospho-RPA2-S4/S8 Rabbit pAb (AP1102) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Overview

Product namePhospho-RPA2-S4/S8 Rabbit pAb
Catalog No.AP1102
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways.
ImmunogenA synthetic phosphorylated peptide around S4 & S8 of human RPA2 (NP_002937.1).
SequenceSGFES
Gene ID
Swiss Prot
SynonymsREPA2; RPA32; RP-A p32; RP-A p34; Phospho-RPA2-S4/S8
Calculated MW29kDa
Observed MW36kDa
ReactivityRat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesC6
Cellular locationNucleus, PML body.
Customer validation

WB(Homo sapiens, Mus musculus)

IHC(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Phospho-RPA2-S4/S8 Rabbit pAb (AP1102)}

Western blot - Phospho-RPA2-S4/S8 Rabbit pAb (AP1102)

Western blot analysis of lysates from C6 cells, using Phospho-RPA2-S4/S8 Rabbit pAb (AP1102) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using AP1102? Please let us know so that we can cite the reference in this datasheet.

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Secondary Antibodies (26)