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RPA2 Rabbit pAb (A24881)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - RPA2 Rabbit pAb (A24881)

Western blot analysis of lysates from HeLa cells using RPA2 Rabbit pAb(A24881) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - RPA2 Rabbit pAb (A24881)

Immunohistochemistry analysis of RPA2 in paraffin-embedded human colon tissue using RPA2 Rabbit pAb (A24881) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - RPA2 Rabbit pAb (A24881)

Immunofluorescence analysis of U-2 OS cells using RPA2 Rabbit pAb(A24881) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRPA2 Rabbit pAb
Catalog No.A24881
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
ImmunogenRecombinant Protein corresponding to a sequence within amino acids 1-270 of human RPA2(NP_002937.1).
SequenceMWNSGFESYGSSSYGGAGGYTQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLVDEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE
Gene ID
Swiss Prot
SynonymsRPA2; REPA2; RP-A p32; RP-A p34; RPA32; RP-Ap32; RP-Ap34; replication protein A2
Calculated MW29kDa/30kDa/38kDa
Observed MW35kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular locationNucleus, PML body

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ABclonal:Western blot - RPA2 Rabbit pAb (A24881)}

Western blot - RPA2 Rabbit pAb (A24881)

Western blot analysis of lysates from HeLa cells using RPA2 Rabbit pAb(A24881) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - RPA2 Rabbit pAb (A24881)}

Immunohistochemistry - RPA2 Rabbit pAb (A24881)

Immunohistochemistry analysis of RPA2 in paraffin-embedded human colon tissue using RPA2 Rabbit pAb (A24881) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - RPA2 Rabbit pAb (A24881)}

Immunofluorescence - RPA2 Rabbit pAb (A24881)

Immunofluorescence analysis of U-2 OS cells using RPA2 Rabbit pAb(A24881) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (4)

Secondary Antibodies (26)