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Phospho-RPS6KA5-S376 Rabbit pAb (AP0800)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-RPS6KA5-S376 Rabbit pAb (AP0800) at 1:2000 dilution. NIH/3T3 cells were treated by Anisomycin (25μg/mL) for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Phospho-RPS6KA5-S376 Rabbit pAb (AP0800)

Immunohistochemistry analysis of paraffin-embedded Rat testis using Phospho-RPS6KA5-S376 Rabbit pAb (AP0800) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using Phospho-RPS6KA5-S376 Rabbit pAb (AP0800) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Phospho-RPS6KA5-S376 Rabbit pAb (AP0800) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product name	Phospho-RPS6KA5-S376 Rabbit pAb
Catalog No.	AP0800
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables ATP binding activity and protein serine/threonine kinase activity. Involved in several processes, including histone-serine phosphorylation; positive regulation of histone modification; and regulation of transcription, DNA-templated. Located in cytoplasm and nucleoplasm.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S376 of human RPS6KA5 (NP_004746.2).
Sequence	GYSFV
Gene ID
Swiss Prot
Synonyms	MSK1; RLPK; MSPK1; Phospho-RPS6KA5-S376
Calculated MW	90kDa
Observed MW	90kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	NIH/3T3 treated by Anisomycin
Cellular location	Cytoplasm, Nucleus.