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Phospho-SMO-S611/615 Rabbit pAb (AP0939)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from Jurkat cells, using Phospho-SMO-S611/615 Rabbit pAb (AP0939) at 1:1000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes. Jurkat cells were treated by Anisomycin (25 μg/mL) at 37℃ for 20 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name	Phospho-SMO-S611/615 Rabbit pAb
Catalog No.	AP0939
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S611 & S615 of human SMO (NP_005622.1).
Sequence	SADVS
Gene ID
Swiss Prot
Synonyms	Gx; CRJS; PHLS; SMOH; FZD11; Phospho-SMO-S611/615
Calculated MW	86kDa
Observed MW	86kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Jurkat treated by Calyculin A
Cellular location	Cell projection, Membrane, Multi-pass membrane protein, cilium.