Phospho-SMO-S616 Rabbit pAb (AP0938)

Datasheet

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

ABclonal:Dot Blot - Phospho-SMO-S616 Rabbit pAb (AP0938)

Dot-blot analysis of all sorts of peptides using Phospho-SMO-S616 antibody (AP0938) at 1:1000 dilution.
Exposure time: 1s.

Overview

Product name	Phospho-SMO-S616 Rabbit pAb
Catalog No.	AP0938
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S616 of human SMO (NP_005622.1).
Sequence	VSSAW
Gene ID
Swiss Prot
Synonyms	Gx; CRJS; PHLS; SMOH; FZD11; Phospho-SMO-S616
Calculated MW	86kDa
Observed MW	Refer to figures

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 DB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples
Cellular location	Cell projection, Membrane, Multi-pass membrane protein, cilium.