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Phospho-STAT5-Y694 Rabbit pAb (AP0887)

Publications (11) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-STAT5-Y694 Rabbit pAb (AP0887)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT5-Y694 Rabbit pAb (AP0887) at 1:400 dilution. NIH/3T3 cells were treated by Pervanadate (1 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Phospho-STAT5-Y694 Rabbit pAb (AP0887)

Immunohistochemistry analysis of paraffin-embedded Rat heart using Phospho-STAT5-Y694 Rabbit pAb (AP0887) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT5-Y694 Rabbit pAb (AP0887)

Immunohistochemistry analysis of paraffin-embedded Human placenta using Phospho-STAT5-Y694 Rabbit pAb (AP0887) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT5-Y694 Rabbit pAb (AP0887)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-STAT5-Y694 Rabbit pAb (AP0887) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product namePhospho-STAT5-Y694 Rabbit pAb
Catalog No.AP0887
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
ImmunogenA synthetic phosphorylated peptide around Y694 of human STAT5 (NP_003143.2/NP_036580.2).
SequenceDGYVK
Gene ID
Swiss Prot
SynonymsMGF; STAT5; stat5a/stat5b; Phospho-STAT5-Y694
Calculated MW90kDa
Observed MW90kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:100 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesNIH/3T3 treated by Pervanadate
Cellular locationcytosol, nucleoplasm.
Customer validation

WB(L. vannamei, Mus musculus, Homo sapiens, Other, Rattus norvegicus)

FC(Mus musculus)

IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Phospho-STAT5-Y694 Rabbit pAb (AP0887)}

    Western blot - Phospho-STAT5-Y694 Rabbit pAb (AP0887)

    Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT5-Y694 Rabbit pAb (AP0887) at 1:400 dilution. NIH/3T3 cells were treated by Pervanadate (1 nM) at 37℃ for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - Phospho-STAT5-Y694 Rabbit pAb (AP0887)}

    Immunohistochemistry - Phospho-STAT5-Y694 Rabbit pAb (AP0887)

    Immunohistochemistry analysis of paraffin-embedded Rat heart using Phospho-STAT5-Y694 Rabbit pAb (AP0887) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-STAT5-Y694 Rabbit pAb (AP0887)}

    Immunohistochemistry - Phospho-STAT5-Y694 Rabbit pAb (AP0887)

    Immunohistochemistry analysis of paraffin-embedded Human placenta using Phospho-STAT5-Y694 Rabbit pAb (AP0887) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-STAT5-Y694 Rabbit pAb (AP0887)}

    Immunohistochemistry - Phospho-STAT5-Y694 Rabbit pAb (AP0887)

    Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-STAT5-Y694 Rabbit pAb (AP0887) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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