Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT5-Y694 Rabbit pAb (AP0887) at 1:400 dilution. NIH/3T3 cells were treated by Pervanadate (1 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat heart using Phospho-STAT5-Y694 Rabbit pAb (AP0887) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta using Phospho-STAT5-Y694 Rabbit pAb (AP0887) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
| Product name | Phospho-STAT5-Y694 Rabbit pAb |
|---|---|
| Catalog No. | AP0887 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around Y694 of human STAT5 (NP_003143.2/NP_036580.2). |
|---|---|
| Sequence | DGYVK |
| Gene ID | |
| Swiss Prot | |
| Synonyms | MGF; STAT5; stat5a/stat5b; Phospho-STAT5-Y694 |
| Calculated MW | 90kDa |
| Observed MW | 90kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry |
| Positive samples | NIH/3T3 treated by Pervanadate |
| Cellular location | cytosol, nucleoplasm. |
| Customer validation | WB(L. vannamei, Mus musculus, Homo sapiens, Other, Rattus norvegicus) FC(Mus musculus) IHC(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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