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STAT5A/STAT5B Rabbit pAb (A17930)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - STAT5A/STAT5B Rabbit pAb (A17930)

Western blot analysis of lysates from mouse kidney, using STAT5A/STAT5B Rabbit pAb (A17930) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameSTAT5A/STAT5B Rabbit pAb
Catalog No.A17930
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 700-787 of human STAT5A/STAT5B (NP_036580.2).
SequenceVKPQIKQVVPEFVNASADAGGGSATYMDQAPSPAVCPQAHYNMYPQNPDSVLDTDGDFDLEDTMDVARRVEELLGRPMDSQWIPHAQS
Gene ID
Swiss Prot
SynonymsMGF; STAT5; stat5a/stat5b; STAT5A/STAT5B
Calculated MW
Observed MW90kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesmouse kidney
Cellular location
    ABclonal:Western blot - STAT5A/STAT5B Rabbit pAb (A17930)}

    Western blot - STAT5A/STAT5B Rabbit pAb (A17930)

    Western blot analysis of lysates from mouse kidney, using STAT5A/STAT5B Rabbit pAb (A17930) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (2)

    Secondary Antibodies (26)