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Phospho-STK3/MST2-T384 Rabbit pAb (AP0935)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-STK3/MST2-T384 Rabbit pAb (AP0935) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	Phospho-STK3/MST2-T384 Rabbit pAb
Catalog No.	AP0935
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T384 of human STK3/MST2 (NP_006272.2).
Sequence	NATSP
Gene ID
Swiss Prot
Synonyms	KRS1; MST2; Phospho-STK3/MST2-T384
Calculated MW	56kDa
Observed MW	54kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by Calyculin A, NIH/3T3 treated by Calyculin A
Cellular location	Cytoplasm, Nucleus.