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Phospho-TBK1/NAK-S172 Rabbit mAb (AP1418)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from HeLa cells using Phospho-TBK1/NAK-S172 Rabbit mAb (AP1418) at 1:1000 dilution incubated overnight at 4℃. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name	Phospho-TBK1/NAK-S172 Rabbit mAb
Catalog No.	AP1418
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC57906

Background

The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. The protein is also an important kinase for antiviral innate immunity response.

Immunogen information

Immunogen	A phospho synthetic peptide corresponding to residues surrounding S172 of Human TBK1/NAK.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	NAK; T2K; IIAE8; FTDALS4; Phospho-TBK1/NAK-S172
Calculated MW	84kDa
Observed MW	84kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa cells treated by Calyculin A
Cellular location	Cytoplasm.
Customer validation	WB(Mus musculus, Rattus norvegicus)