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Phospho-TIFA-T9 Rabbit mAb (AP1462)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from TIFA knockout (KO) 293T cells transfected with TIFA or mutational TIFA, using Phospho-TIFA-T9 Rabbit mAb (AP1462) at 1:5000 dilution, 293T cells were treated by ADP-heptose.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Review

Overview

Product name	Phospho-TIFA-T9 Rabbit mAb
Catalog No.	AP1462
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC66052

Background

This gene encodes an adapter protein involved in adaptive and innate immunity. This protein includes a forkhead-associated (FHA) domain that specifically binds to phosphorylated serine and threonine residues. In response to bacterial infection, the encoded host cell protein undergoes an intermolecular interaction between the FHA domain and a phosphorylated threonine that leads to protein oligomerization and stimulation of the NF-kappa B and other downstream signaling pathways. This protein exhibits reduced expression in hepatocellular carcinoma and may suppress hepatocellular carcinoma progression. This protein may also play a role in the DNA damage response.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T9 of human TIFA(NP_443096.1).
Sequence	ADTEE
Gene ID
Swiss Prot
Synonyms	T2BP; T6BP; TIFAA; Phospho-TIFA-T9
Calculated MW	21kDa
Observed MW	20kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:6000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	TIFA knockout (KO) 293T treated by ADP-heptose after transfected with human TIFA
Cellular location	Cytoplasm.
Customer validation	(NALM-6 cells, HEK293T cells)