Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat
Western blot analysis of various lysates using Phospho-VANGL2-S79/S82/S84 Rabbit mAb (AP1207) at1:1000 dilution. HeLa and C6 cells were treated by wnt(100ng/ml) for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
| Product name | Phospho-VANGL2-S79/S82/S84 Rabbit mAb |
|---|---|
| Catalog No. | AP1207 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC5022 |
| Immunogen | A phospho specific peptide corresponding to residues surrounding S79/S82/S84 of human VANGL2 |
|---|---|
| Sequence | GTSEHSISHD |
| Gene ID | |
| Swiss Prot | |
| Synonyms | LPP1; LTAP; STB1; STBM; STBM1; Phospho-VANGL2-S79/S82/S84 |
| Calculated MW | 60kDa |
| Observed MW | 65kDa |
| Reactivity | Human, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HeLa treated by wnt, C6 treated by wnt |
| Cellular location | Cell membrane, Multi-pass membrane protein. |
| Customer validation | WB(Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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