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Phospho-VANGL2-T78/S79/S82 Rabbit mAb (AP1206)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-VANGL2-T78/S79/S82 Rabbit mAb (AP1206) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by wnt(100ng/ml) for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name	Phospho-VANGL2-T78/S79/S82 Rabbit mAb
Catalog No.	AP1206
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC5008-03

Background

The protein encoded by this gene is a membrane protein involved in the regulation of planar cell polarity, especially in the stereociliary bundles of the cochlea. The encoded protein transmits directional signals to individual cells or groups of cells in epithelial sheets. This protein is also involved in the development of the neural plate.

Immunogen information

Immunogen	A phospho specific peptide corresponding to residues surrounding T78/S79/S82 of human VANGL2
Sequence	TSEHS
Gene ID
Swiss Prot
Synonyms	LPP1; LTAP; STB1; STBM; STBM1; Phospho-VANGL2-T78/S79/S82
Calculated MW	60kDa
Observed MW	60kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by wnt, NIH/3T3 treated by wnt, C6 treated by wnt
Cellular location	Cell membrane, Multi-pass membrane protein.
Customer validation	WB(Homo sapiens)