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Phospho-VASP-S157 Rabbit pAb (AP0763)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HEL cells using Phospho-VASP-S157 Rabbit pAb (AP0763) at 1:1000 dilution. HEL cells were treated by Forskolin (20 uM) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.8s.

ABclonal:Immunohistochemistry - Phospho-VASP-S157 Rabbit pAb (AP0763)

Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-VASP-S157 Rabbit pAb (AP0763) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-VASP-S157 Rabbit pAb (AP0763) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse lung using Phospho-VASP-S157 Rabbit pAb (AP0763) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunoprecipitation - Phospho-VASP-S157 Rabbit pAb (AP0763)

Immunoprecipitation analysis of 200 μg extracts of A-431 cells, using 3 μg Phospho-VASP-S157 pAb (AP0763). Western blot was performed from the immunoprecipitate using Phospho-VASP-S157 pAb (AP0763) at a dilution of 1:1000. A-431 cells were treated by Forskolin (10 uM) at 37℃ for 30 minutes after serum-starvation overnight.

Overview

Product name	Phospho-VASP-S157 Rabbit pAb
Catalog No.	AP0763
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S157 of human Phospho-VASP-S157 (NP_003361.1).
Sequence	RVSNA
Gene ID
Swiss Prot
Synonyms	VASP; Phospho-VASP-S157
Calculated MW	40kDa
Observed MW	50kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:100 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	HEL treated by Forskolin
Cellular location	Cell junction, Cell projection, Cytoplasm, cytoskeleton, filopodium membrane, focal adhesion, lamellipodium membrane, tight junction.