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Phospho-VASP-S239 Rabbit mAb (AP1478)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from HEL cells using Phospho-VASP-S239 Rabbit mAb (AP1478) at 1:3000 dilution. HEL cells were treated by Forskolin (20 uM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - Phospho-VASP-S239 Rabbit mAb (AP1478)

Confocal imaging of HEL (treated with Forskolin) and HEL (untreated) cells using Phospho-VASP-S239 Rabbit mAb (AP1478, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name	Phospho-VASP-S239 Rabbit mAb
Catalog No.	AP1478
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC65969

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S239 of human VASP (NP_003361.1).
Sequence	KVSKQE
Gene ID
Swiss Prot
Synonyms	VASP; vasodilator-stimulated phosphoprotein; Phospho-VASP-S239
Calculated MW	40kDa
Observed MW	52kDa/56kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HEL+Forskolin
Cellular location	Plasma membrane, Cytoplasm, Nucleus