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Phospho-Zap70-Y319+Syk-Y352 Rabbit pAb (AP1497)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Immunofluorescence - Phospho-Zap70-Y319+Syk-Y352 Rabbit pAb (AP1497)

Confocal imaging of EL4 cells (treated with H₂O₂ and pervanadate) and EL4 cells (untreated) using Phospho-Zap70-Y319+Syk-Y352 Rabbit pAb (AP1497, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product namePhospho-Zap70-Y319+Syk-Y352 Rabbit pAb
Catalog No.AP1497
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around Y319 of human ZAP70 (NP_001070.2).
SequenceSPY(p)SD
Gene ID
Swiss Prot
SynonymsSRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
Calculated MW70kDa
Observed MWRefer to figures
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples
Cellular locationCell membrane, Cytoplasm, Peripheral membrane protein.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunofluorescence - Phospho-Zap70-Y319+Syk-Y352 Rabbit pAb (AP1497)}

    Immunofluorescence - Phospho-Zap70-Y319+Syk-Y352 Rabbit pAb (AP1497)

    Confocal imaging of EL4 cells (treated with H₂O₂ and pervanadate) and EL4 cells (untreated) using Phospho-Zap70-Y319+Syk-Y352 Rabbit pAb (AP1497, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)