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Phospho-eIF2α-S51 Rabbit pAb (AP0342)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of various lysates using Phospho-eIF2α-S51 Rabbit pAb (AP0342) at 1:1000 dilution. HeLa and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Review

Overview

Product name	Phospho-eIF2α-S51 Rabbit pAb
Catalog No.	AP0342
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S51 of human EIF2S1 (NP_004085.1).
Sequence	SELSR
Gene ID
Swiss Prot
Synonyms	EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; Phospho-eIF2α-S51
Calculated MW	36kDa
Observed MW	38kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by Calyculin A, C6 treated by Calyculin A
Cellular location	Cytoplasmic granule.
Customer validation	(Raw264.7, HeLa, HepG2) WB(Homo sapiens, Mus musculus)