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Podoplanin Rabbit pAb (A13261)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Podoplanin Rabbit pAb (A13261)

Western blot analysis of various lysates using Podoplanin Rabbit pAb (A13261) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Podoplanin Rabbit pAb (A13261)

Immunohistochemistry analysis of paraffin-embedded Human colon using Podoplanin Rabbit pAb (A13261) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Podoplanin Rabbit pAb (A13261)

Immunohistochemistry analysis of paraffin-embedded Human esophagus using Podoplanin Rabbit pAb (A13261) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product namePodoplanin Rabbit pAb
Catalog No.A13261
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 23-131 of human Podoplanin (NP_006465.3).
SequenceASTGQPEDDTETTGLEGGVAMPGAEDDVVTPGTSEDRYKSGLTTLVATSVNSVTGIRIEDLPTSESTVHAQEQSPSATASNVATSHSTEKVDGDTQTTVEKDGLSTVTL
Gene ID
Swiss Prot
SynonymsT1A; GP36; GP40; Gp38; OTS8; T1A2; TI1A; D2-40; T1A-2; AGGRUS; HT1A-1; PA2.26; Podoplanin
Calculated MW17kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:20 - 1:50
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse lung, Rat kidney
Cellular locationCell projection, Membrane, Single-pass type I membrane protein, filopodium membrane, lamellipodium membrane, microvillus membrane, ruffle membrane.
Customer validation

WB(Rattus norvegicus, Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - Podoplanin Rabbit pAb (A13261)}

Western blot - Podoplanin Rabbit pAb (A13261)

Western blot analysis of various lysates using Podoplanin Rabbit pAb (A13261) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Podoplanin Rabbit pAb (A13261)}

Immunohistochemistry - Podoplanin Rabbit pAb (A13261)

Immunohistochemistry analysis of paraffin-embedded Human colon using Podoplanin Rabbit pAb (A13261) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Podoplanin Rabbit pAb (A13261)}

Immunohistochemistry - Podoplanin Rabbit pAb (A13261)

Immunohistochemistry analysis of paraffin-embedded Human esophagus using Podoplanin Rabbit pAb (A13261) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A13261? Please let us know so that we can cite the reference in this datasheet.

항체 (7)

재조합 단백질 (1)

Secondary Antibodies (26)